Date of Award

1-1-2011

Language

English

Document Type

Dissertation

Degree Name

Doctor of Philosophy (PhD)

College/School/Department

Department of Chemistry

Content Description

1 online resource (viii, 111 pages) : illustrations (some color)

Dissertation/Thesis Chair

Jason R Shepard

Committee Members

Igor Lednev, Carla Theimer, Paul Toscano, Hua Shi

Keywords

Cells, Cytology, Cell transplantation

Subject Categories

Analytical Chemistry | Biochemistry

Abstract

In the past decade, the shift from whole cell population analyses towards single cell measurement methods and techniques is based on experimental results that reveal significant levels of non-genetic heterogeneity in clonal cell populations. This heterogeneity manifests in multiple aspects of cell activity and is, in part, a result of stochastic noise in processes leading to gene expression, namely transcription and translation. The growing understanding of this occurrence has led to the development of methods to monitor and analyze heterogeneity for a more thorough description of cell populations and overall activity.

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