Date of Award

1-1-2018

Language

English

Document Type

Dissertation

Degree Name

Doctor of Philosophy (PhD)

College/School/Department

Department of Chemistry

Content Description

1 online resource (ii, xvi, 189 pages) : color illustrations.

Dissertation/Thesis Chair

Mehmet V Yigit

Committee Members

Alexander Shekhtman, Jan Halamek, Jia Sheng, Ken Halvorsen

Keywords

array, DNA, miRNA, nanoparticle, sensor, two-dimensional, Oligonucleotides, Biosensors, Nanobiotechnology, Fluorimetry, Molecular diagnosis

Subject Categories

Biochemistry | Chemistry | Materials Science and Engineering

Abstract

Nucleic acid technology along with vast variety of nanomaterials has demonstrated a great potential in many applications from biosensing studies to molecular diagnostics, from biomedical and bioanalytical research to environmental analysis. Especially short single stranded (ss) DNA molecules, called oligonucleotides, are extraordinary biopolymers featuring diverse functionality on the nanoparticles thanks to their high degree of programmability, target-specific binding or cleavage, molecular recognition ability, structure-switching capability, and unique interactions at the bio-nano interfaces. Among those, there have been many biosensing applications utilizing ss DNAs and numerous nanomaterials through various detection techniques such as fluorometric, colorimetric or electrochemical methods. Although many groundbreaking applications have been carried out, some challenges arising from sensitivity or selectivity issues remain to be addressed, especially for molecular diagnostics. Functional sensory systems with cost-effective, rapid, and easy-to-operate layouts, which are crucial for point-of-care diagnostics, are still an unmet need for certain fields to target disease-related critical biomarkers such as proteins, cells, circulating DNAs or miRNAs.

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