Comparative phase imaging of live cells by digital holographic microscopy and transport of intensity equation methods

Authors

Jeremy M. Wittkopp, University at Albany, State University of New York
Ting Chean Khoo, University at Albany, State University of New York
Shane Carney, University at Albany, State University of New York
Kai Pisila, University at Albany, State University of New York
Shahab J. Bahreini, University at Albany, State University of New York
Kate Tubbesing, University at Albany, State University of New York
Anna Sharikova, University at Albany, State University of New York
Alexander Khmaladze, University at Albany, State University of New York
Supriya Mahajan, Albany Medical College

Document Type

Article

Publication Date

2020

DOI

https://doi.org/10.1364/OE.385854

Abstract

We describe a microscopic setup implementing phase imaging by digital holographic microscopy (DHM) and transport of intensity equation (TIE) methods, which allows the results of both measurements to be quantitatively compared for either live cell or static samples. Digital holographic microscopy is a well-established method that provides robust phase reconstructions, but requires a sophisticated interferometric imaging system. TIE, on the other hand, is directly compatible with bright-field microscopy, but is more susceptible to noise artifacts. We present results comparing DHM and TIE on a custom-built microscope system that allows both techniques to be used on the same cells in rapid succession, thus permitting the comparison of the accuracy of both methods.

Comments

This is the Publisher’s PDF of the following article made available by Optica Publishing Group: Jeremy M. Wittkopp, Ting Chean Khoo, Shane Carney, Kai Pisila, Shahab J. Bahreini, Kate Tubbesing, Supriya Mahajan, Anna Sharikova, Jonathan C. Petruccelli, and Alexander Khmaladze, "Comparative phase imaging of live cells by digital holographic microscopy and transport of intensity equation methods," Opt. Express 28, 6123-6133 (2020)

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Recommended Citation

Wittkopp, Jeremy M.; Khoo, Ting Chean; Carney, Shane; Pisila, Kai; Bahreini, Shahab J.; Tubbesing, Kate; Sharikova, Anna; Khmaladze, Alexander; and Mahajan, Supriya, "Comparative phase imaging of live cells by digital holographic microscopy and transport of intensity equation methods" (2020). Physics Faculty Scholarship. 53.
https://scholarsarchive.library.albany.edu/physics_fac_scholar/53