Date of Award

1-1-2013

Language

English

Document Type

Master's Thesis

Degree Name

Master of Science (MS)

College/School/Department

Department of Chemistry

Content Description

1 online resource (vii, 70 pages) : illustrations (some color)

Dissertation/Thesis Chair

Daniele Fabris

Committee Members

Alexander Shekhtman

Keywords

Mass Spectrometry, Post-Transcriptional Modification, RNA, HIV (Viruses), Mass spectrometry, Nucleosides

Subject Categories

Chemistry

Abstract

The genomic structure of the human immunodeficiency virus type 1 (HIV-1) has proven difficult to examine by traditional high-resolution techniques. Structural probing and in silico modeling have been turned to the task instead. Computer models are only as robust as the data used to create them. A number of techniques were explored in this investigation to expand the utility of in silico modeling as it relates to HIV-1: the study of nucleoside modifications, and the probing of in vivo conformations of the 5'-untranslated region (5'-UTR) by cross-linking and affinity capture.

Included in

Chemistry Commons

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