Date of Award

1-1-2012

Language

English

Document Type

Dissertation

Degree Name

Doctor of Philosophy (PhD)

College/School/Department

Department of Biomedical Sciences

Content Description

1 online resource (xiii, 185 pages) : color illustrations.

Dissertation/Thesis Chair

Randall H Morse

Committee Members

M. Joan Curcio, Steven D Hanes, Richard S Zitomer, Thomas J Begley

Keywords

Saccharomyces cerevisiae, Transcription factors, Genetic transcription, Chromatin

Subject Categories

Genetics | Molecular Biology

Abstract

Abf1 and Rap1 are functionally similar general regulatory factors (GRFs) found in Saccharomyces cerevisiae . Abf1, in its role as a transcriptional activator, exerts a memory effect on some genes under its control. This effect results in transcription levels remaining steady when Abf1 dissociates from its binding site in a conditional mutant. In contrast, Rap1 fails to elicit the same effect on its regulatory targets. Transcriptional memory effects have been observed in many fields of study, including immunology, cancer, and stem cells, and conservation of transcription machinery will allow studies in yeast to be applied to higher organisms.

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