The role of ESX1 in secretion and sex-determination of Mycobacterium smegmatis

Date of Award

1-1-2012

Language

English

Document Type

Dissertation

Degree Name

Doctor of Philosophy (PhD)

College/School/Department

Department of Biomedical Sciences

Content Description

1 online resource (x, 171 pages) : illustrations (some color)

Dissertation/Thesis Chair

Keith M Derbyshire

Committee Members

April Burch, M. Joan Curcio, Robert Osuna, Gary Winslow

Keywords

conjugation, secretion, Genetic sex determination, Mycobacterium tuberculosis, Proteins, Transcription factors

Subject Categories

Microbiology | Molecular Biology

Abstract

The ESX-1 secretory apparatus is a complex molecular machine with multiple components and substrates. I have conducted experiments to identify protein substrates of ESX-1 and to dissect the role of ESX-1 in conjugation. HA-fusion constructs of M. smegmatis genes associated with ESX-1 function were assessed by western blot for secretion. SaeAMs, EspHMs, EspBMs, and Ms0077 are secreted from mc2155. While McLaughlin et al. (2007) previously reported the secretion of EspBMm, the secretion of SaeAMs, EspHMs, EspHMt, and Ms0077 are novel findings.

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