Date of Award
1-1-2009
Language
English
Document Type
Dissertation
Degree Name
Doctor of Philosophy (PhD)
College/School/Department
Department of Biomedical Sciences
Content Description
1 online resource (vii, 128 pages) : illustrations (some color)
Dissertation/Thesis Chair
Joachim Frank
Committee Members
Rajendra Agrawal, Paul Masters, Terry Wagenknecht, Jacek Wower
Keywords
cryo-electron microscopy, ribosome, single-particle reconstruction, transfer-messenger RNA, transloaction, Proteins, Translocation (Genetics), Transfer RNA, Ribosomes
Subject Categories
Biophysics | Molecular Biology
Abstract
Single-particle reconstruction from cryo-electron microscopy (cryo-EM) images is a widely used technique in studying the structure of macromolecular complexes. This technique allows the visualization of molecules in their native hydrated state. The heterogeneity of two-dimensional projection images, resulting from the co-existence of different states of a macromolecular complex, remains a major obstacle, as it impairs the validity of reconstruction and limits the progress toward higher resolution. On the other hand, co-existing structures, if successfully extracted from a single dataset, can provide valuable information about the equilibrium and dynamics of the biological system being studied.
Recommended Citation
Fu, Jie, "Study of dynamic processes in protein synthesis using heterogeneous ribosome specimens" (2009). Legacy Theses & Dissertations (2009 - 2024). 38.
https://scholarsarchive.library.albany.edu/legacy-etd/38