Date of Award
1-1-2022
Language
English
Document Type
Master's Thesis
Degree Name
Master of Science (MS)
College/School/Department
Department of Biological Sciences
Content Description
1 online resource (v, 34 pages) : illustrations (some color)
Dissertation/Thesis Chair
Gabriele Fuchs
Committee Members
Cara T Pager, Alex M Valm
Keywords
lysine acetylation, lysine acetyltransferase, poliovirus, ribosomal protein, Ribosomes, Poliovirus, Betaine, Acetylation
Subject Categories
Molecular Biology | Virology
Abstract
Ribosomes have been traditionally viewed as invariable machinery, but in recent years more and more evidence for their heterogeneity has emerged. Core ribosomal proteins, ribosomal-associated proteins, and post-translational modifications all contribute to the variations found within these crucial subcellular components.In our research, we focused on lysine acetylation – a post translational modification found on many ribosomal proteins. We used a chemical inhibitor of acetyltransferases KAT 3A and KAT 3B to observe the effect that inhibiting acetylation may have in the context of translation. By using poliovirus to shut off conventional cellular translation, we were able to look at a narrow slice of translation – namely, IRES-mediated viral translation in response to acetylation inhibition. We used a combination of experiments, from plaque assays for broader phenotypic observation of KAT3 inhibition, to DNA and RNA transfections, and 35S metabolic assays for a closer focus on specific aspects of the viral life cycle.
Recommended Citation
Norkvests, Eduards, "Inhibition of lysine acetyltransferases KAT 3A/3B and its effect on poliovirus proliferation" (2022). Legacy Theses & Dissertations (2009 - 2024). 2983.
https://scholarsarchive.library.albany.edu/legacy-etd/2983