Date of Award

1-1-2010

Language

English

Document Type

Dissertation

Degree Name

Doctor of Philosophy (PhD)

College/School/Department

Department of Environmental Health Sciences

Content Description

1 online resource (xii, 120 pages) : illustrations (some color)

Dissertation/Thesis Chair

Xinxin Ding

Committee Members

Laurence Kaminsky, David Spink, Brian Pentecost, Bruce Herron

Keywords

CPR(POR), CYP2 gene cluster, CYP2A13, Cytochrome P450, knockout mouse models, transgenic mouse models, Cytochrome P-450, Transgenic mice, Animal models in research

Subject Categories

Environmental Health | Molecular Biology

Abstract

The overall objective of this dissertation is to study the in vivo function of microsomal cytochrome P450 monooxygenases (P450s), which metabolize numerous drugs, chemical carcinogens, environmental pollutants, as well as endogenous signaling molecules such as steroid hormones and eicosanoids. The major research tool of this study involves the development of transgenic and knockout mouse models. The specific aims are 1) to study the in vivo function of NADPH-cytochrome P450 reductase (CPR) and CPR-dependent enzymes using a mouse model with a reversible hypomorphic Cpr gene; 2) to study the in vivo function of CYP2A13 with a CYP2A13- transgenic model; and 3) to generate a novel Cyp2a-2b-2f-2g-2s-null mouse model as the background strain for CYP2A13-humanized model.

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