Date of Award

12-1-2020

Language

English

Document Type

Dissertation

Degree Name

Doctor of Philosophy (PhD)

College/School/Department

Department of Biological Sciences

Content Description

1 online resource (viii, 241 pages) : illustrations (chiefly color)

Dissertation/Thesis Chair

Ben G. Szaro

Committee Members

Melinda Larsen, Cara Pager, Scott Tenebaum, Morgan Sammons

Keywords

Axon, Central Nervous System, Regeneration, RNA-seq, Xenopus laevis, Axons, Central nervous system, Gene expression

Subject Categories

Biology

Abstract

Background: The South African claw-toed frog, Xenopus laevis, is uniquely suited for studying differences between regenerative and non-regenerative responses to CNS injury within the same organism, because some CNS neurons (e.g., retinal ganglion cells after optic nerve crush (ONC)) regenerate axons throughout life, whereas others (e.g., hindbrain neurons after spinal cord injury (SCI)) lose this capacity as tadpoles metamorphose into frogs. Tissues from these CNS regions (frog ONC eye, tadpole SCI hindbrain, frog SCI hindbrain) were used in a three-way RNA-seq study of axotomized CNS axons to identify potential core gene expression programs for successful CNS axon regeneration.

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