Date of Award

1-1-2019

Language

English

Document Type

Dissertation

Degree Name

Doctor of Philosophy (PhD)

College/School/Department

Department of Biological Sciences

Content Description

1 online resource (xix, 320 pages) : color illustrations.

Dissertation/Thesis Chair

Marlene Belfort

Committee Members

Richard Cunningham, Kathleen McDonough, Robert Osuna, Sudha Chaturvedi

Keywords

crystal structure, inteins, metals, protein splicing, Eukaryotic cells, Protein precursors, Protein engineering, Pathogenic microorganisms, Microbial genomics

Subject Categories

Biochemistry | Bioinformatics | Biology

Abstract

Inteins are self-splicing elements that orchestrate the autocatalytic process of protein splicing, during which the intein excises itself from a host polypeptide. This multistep reaction involves a series of coordinated nucleophilic attacks and peptide bond rearrangements that remove the intein and reassemble the flanking halves, called exteins, to form the mature host protein. Some inteins are also mobile elements, and can spread to the same or ectopic sites using an internal homing endonuclease domain.

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