Imaging approaches to study neuron and astrocyte morphology

Author

Lianna Yin D'Brant, University at Albany, State University of New YorkFollow

Date of Award

1-1-2018

Language

English

Document Type

Master's Thesis

Degree Name

Master of Science (MS)

College/School/Department

Department of Physics

Content Description

1 online resource (x, 95 pages) : illustrations.

Dissertation/Thesis Chair

Alexander Khmaladze

Committee Members

Carolyn MacDonald, Jonathan Petruccelli

Keywords

apoptosis, EAAC1, microscopy, Neurons, Astrocytes, Confocal microscopy, Digital holographic microscopy, Raman spectroscopy, Nanotechnology, Brain, Cell membranes, Microscopy, Confocal, Spectrum Analysis, Raman, Cell Membrane, brains

Subject Categories

Neuroscience and Neurobiology | Physics

Abstract

In this research work, we applied several imaging techniques to study the morphology of brain cells in tissues. More specifically, we applied confocal microscopy, digital holography, three dimensional tomographic nanoscopy and Raman microspectroscopy to detect changes in neurons, astrocytes and C6 glioma cells. We quantified cell swelling in brain sections of the mouse striatum and hippocampus by measuring changes in the index of refraction, which eliminated the need for dye staining of the samples.

Comments

Requested ProQuest takedown; no end date

Recommended Citation

D'Brant, Lianna Yin, "Imaging approaches to study neuron and astrocyte morphology" (2018). Legacy Theses & Dissertations (2009 - 2024). 2036.
https://scholarsarchive.library.albany.edu/legacy-etd/2036