Date of Award

1-1-2017

Language

English

Document Type

Dissertation

Degree Name

Doctor of Philosophy (PhD)

College/School/Department

Department of Nanoscale Science and Engineering

Program

Nanoscale Engineering

Content Description

1 online resource (x, 117 pages) : color illustrations.

Dissertation/Thesis Chair

James Castracane

Committee Members

Melinda Larsen, Natalya Tokranova, Madhubhani Hemachandra, Yubing Xie

Keywords

Electrospun fibers, Growth factor delivery, Salivary glands, Tissue regeneration, Biomimetic materials, Tissue scaffolds, Nanofibers, Nanobiotechnology

Subject Categories

Chemistry | Nanoscience and Nanotechnology

Abstract

Development of electrospun nanofibers that mimic the structural, mechanical and biochemical properties of natural extracellular matrices (ECMs) and the basement membranes is a promising approach for tissue regeneration. Electrospun fibers of synthetic polymers partially mimic the topography of the ECM, however, their high stiffness, poor hydrophilicity and lack of in vivo-like biochemical cues is not optimal for epithelial cell self-organization and function. In search of a biomimetic scaffold for salivary gland tissue regeneration, we utilized proteins and peptides to induce biomechanical and biochemical cues that resembles the native tissue environment. These functional molecules were introduced to our previously-developed poly lactic-co-glycolic acid (PLGA) nanofiber scaffold by bend electrospinning, covalent conjugation and double emulsion electrospinning. A ductal cell line, submandibular immortalized mouse salivary cells (SIMS) were tested on the proposed scaffolds.

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