Date of Award

1-1-2016

Language

English

Document Type

Master's Thesis

Degree Name

Master of Science (MS)

College/School/Department

Department of Chemistry

Content Description

1 online resource (ii, 39 pages) : illustrations (some color)

Dissertation/Thesis Chair

Alexander Shekhtman

Committee Members

Maksim Royzen

Keywords

Diaphanous-1, GFP, HeLa Cells, In-cell NMR, RAGE, Nuclear magnetic resonance spectroscopy, Protein binding, Glycosylation, Diabetes

Subject Categories

Biochemistry | Chemistry

Abstract

We developed a new technology to study protein-protein interaction in mammalian cells. This technology is based high resolution of Nucleic Magnetic Resonance (NMR) spectroscopy. Using this technology we studied interaction between the receptor for advanced glycation endproducts (RAGE). RAGE- is a multiligand receptor of immunoglobulin receptor family that is activated by a multitude of ligands. Activation of RAGE results in signal transduction that leads to the inflammatory response implicated in the complications of Diabetes. RAGE is an emergent drug target that has been explored for the variety of pathologist including cancers, neurological disorders, inflammatory disease, and diabetes. and Intracellular effector Diaphanous-1 involved in complications of Diabetes. This In-cell NMR technology holds promise for studying a vast array of pharmacologically important interactions in the development of a new generation of effective therapies.

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