Date of Award

1-1-2014

Language

English

Document Type

Dissertation

Degree Name

Doctor of Philosophy (PhD)

College/School/Department

Department of Biomedical Sciences

Content Description

1 online resource (xix, 255 pages) : illustrations (some color)

Dissertation/Thesis Chair

M Joan Curcio

Committee Members

Thomas Begley, Douglas Conklin, Randall Morse, Scott A Tenenbaum, Joseph T Wade

Keywords

Paralog, Retrotransposon, RPL7A, RPL7B, Ty1, Microbiology, Retroviruses, Ribosomes, Genetic translation

Subject Categories

Biology | Genetics | Molecular Biology

Abstract

Replication of the Ty1 retrovirus-like transposon of the yeast Saccharomyces cerevisiae is stringently regulated to reduce the frequency of deleterious retrotransposition events. However, under stress conditions, Ty1 retrotransposition can lead to adaptive genomic alterations. To characterize host regulation of Ty1 retrotransposition, I analyzed ribosome profiling data and showed that Ty1 RNA is efficiently translated. Moreover, the ribosome biogenesis factors BUD21, DBP7, HCR1, LOC1, MRT4, and PUF6 are required for optimal expression of the Ty1 protein Gag.

Download

Share

COinS