Sequential Regulation of Maternal mRNAs through a Conserved cis-Acting Element in Their 3′ UTRs

Authors

Gabriele Fuchs, University at Albany, State University of New York
Pooja Flora, University at Albany, State University of New York
Siu Wah Wong-Deyrup, University at Albany, State University of New York
Elliot Todd Martin, University at Albany, State University of New York
Ryan J. Palumbo, University at Albany, State University of New York
Mohamad Nasrallah, University at Albany, State University of New York
Andrew Oligney, University at Albany, State University of New York
Patrick Blatt, University at Albany, State University of New York
Dhruv Patel, University at Albany, State University of New York
Prashanth Rangan, University at Albany, State University of New York

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Document Type

Article

Publication Date

12-2018

DOI

https://doi.org/10.1016/j.celrep.2018.12.007

Abstract

Maternal mRNAs synthesized during oogenesis initiate the development of future generations. Some maternal mRNAs are either somatic or germline determinants and must be translationally repressed until embryogenesis. However, the translational repressors themselves are temporally regulated. We used polar granule component (pgc), a Drosophila maternal mRNA, to ask how maternal transcripts are repressed while the regulatory landscape is shifting. pgc, a germline determinant, is translationally regulated throughout oogenesis. We find that different conserved RNA-binding proteins bind a 10-nt sequence in the 3′ UTR of pgc mRNA to continuously repress translation at different stages of oogenesis. Pumilio binds to this sequence in undifferentiated and early-differentiating oocytes to block Pgc translation. After differentiation, Bruno levels increase, allowing Bruno to bind the same sequence and take over translational repression of pgc mRNA. We have identified a class of maternal mRNAs that are regulated similarly, including zelda, the activator of the zygotic genome.

Comments

This is the Publisher’s PDF of the following article made available by Cell Reports:

https://doi.org/10.1016/j.celrep.2018.12.007

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.

Recommended Citation

Fuchs, Gabriele; Flora, Pooja; Wong-Deyrup, Siu Wah; Martin, Elliot Todd; Palumbo, Ryan J.; Nasrallah, Mohamad; Oligney, Andrew; Blatt, Patrick; Patel, Dhruv; and Rangan, Prashanth, "Sequential Regulation of Maternal mRNAs through a Conserved cis-Acting Element in Their 3′ UTRs" (2018). Biological Sciences Faculty Scholarship. 27.
https://scholarsarchive.library.albany.edu/biology_fac_scholar/27