Establishment of a Murine Pro-acinar Cell Line to Characterize Roles for FGF2 and α3β1 Integrins in Regulating Pro-acinar Characteristics

Authors

Renée F. Thiemann, Albany Medical College
Deirdre A. Nelson, University at Albany, State University of New YorkFollow
C. Michael DiPersio, Albany Medical Center
Melinda Larsen, University at Albany, State University of New YorkFollow
Susan E. LaFlamme, Albany Medical College

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Document Type

Article

Publication Date

7-29-2019

DOI

10.1038/s41598-019-47387-y

Abstract

Radiation therapy for head and neck cancers results in permanent damage to the saliva producing acinar compartment of the salivary gland. To date, a pure pro-acinar cell line to study underlying mechanisms of acinar cell diferentiation in culture has not been described. Here, we report the establishment of a pro-acinar (mSG-PAC1) and ductal (mSG-DUC1) cell line, from the murine submandibular salivary gland (SMG), which recapitulate developmental milestones in diferentiation. mSG-DUC1 cells express the ductal markers, keratin-7 and keratin-19, and form lumenized spheroids. mSG-PAC1 cells express the pro-acinar markers SOX10 and aquaporin-5. Using the mSG-PAC1 cell line, we demonstrate that FGF2 regulates specifc steps during acinar cell maturation. FGF2 up-regulates aquaporin-5 and the expression of the α3 and α6 subunits of the α3β1 and α6β1 integrins that are known to promote SMG morphogenesis and diferentiation. mSG-DUC1 and mSG-PAC1 cells were derived from genetically modifed mice, homozygous for foxed alleles of the integrin α3 subunit. Similar to SMGs from α3-null mice, deletion of α3 alleles in mSG-PAC1 cells results in the up-regulation of E-cadherin and the downregulation of CDC42. Our data indicate that mSG-DUC1 and mSG-PAC1 cells will serve as important tools to gain mechanistic insight into salivary gland morphogenesis and diferentiation.

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Publisher Acknowledgement:

This is the Publisher's PDF version. The version of record appears here: Thiemann, R.F., Nelson, D.A., Michael DiPersio, C. et al. Establishment of a Murine Pro-acinar Cell Line to Characterize Roles for FGF2 and α3β1 Integrins in Regulating Pro-acinar Characteristics. Sci Rep 9, 10984 (2019). https://doi.org/10.1038/s41598-019-47387-y

Recommended Citation

Thiemann, Renée F.; Nelson, Deirdre A.; DiPersio, C. Michael; Larsen, Melinda; and LaFlamme, Susan E., "Establishment of a Murine Pro-acinar Cell Line to Characterize Roles for FGF2 and α3β1 Integrins in Regulating Pro-acinar Characteristics" (2019). Biological Sciences Faculty Scholarship. 11.
https://scholarsarchive.library.albany.edu/biology_fac_scholar/11