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Title

Mesenchymal Cells Affect Salivary Epithelial Cell Morphology on PGS/PLGA Core/Shell Nanofibers

Authors

Lauren Sfakis, SUNY Polytechnic Institute
Tim Kamaldinov, SUNY Polytechnic Institute
Alexander Khmaladze, University at Albany, State University of New YorkFollow
Zeinab F. Hosseini, University at Albany, State University of New York
Deirdre A. Nelson, University at Albany, State University of New York
Melinda Larsen, University at Albany, State University of New YorkFollow
James Castracane, SUNY Polytechnic Institute

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Document Type

Article

Publication Date

3-29-2018

DOI

10.3390/ijms19041031

Abstract

Engineering salivary glands is of interest due to the damaging effects of radiation therapy and the autoimmune disease Sjögren’s syndrome on salivary gland function. One of the current problems in tissue engineering is that in vitro studies often fail to predict in vivo regeneration due to failure of cells to interact with scaffolds and of the single cell types that are typically used for these studies. Although poly (lactic co glycolic acid) (PLGA) nanofiber scaffolds have been used for in vitro growth of epithelial cells, PLGA has low compliance and cells do not penetrate the scaffolds. Using a core-shell electrospinning technique, we incorporated poly (glycerol sebacate) (PGS) into PLGA scaffolds to increase the compliance and decrease hydrophobicity. PGS/PLGA scaffolds promoted epithelial cell penetration into the scaffold and apical localization of tight junction proteins, which is necessary for epithelial cell function. Additionally, co-culture of the salivary epithelial cells with NIH3T3 mesenchymal cells on PGS/PLGA scaffolds facilitated epithelial tissue reorganization and apical localization of tight junction proteins significantly more than in the absence of the mesenchyme. These data demonstrate the applicability of PGS/PLGA nanofibers for epithelial cell self-organization and facilitation of co-culture cell interactions that promote tissue self-organization in vitro.

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Publisher Acknowledgement:

This is the Publisher's PDF version. The version of record appears here:

Sfakis L, Kamaldinov T, Khmaladze A, Hosseini ZF, Nelson DA, Larsen M, Castracane J. Mesenchymal Cells Affect Salivary Epithelial Cell Morphology on PGS/PLGA Core/Shell Nanofibers. International Journal of Molecular Sciences. 2018; 19(4):1031. https://doi.org/10.3390/ijms19041031

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Recommended Citation

Sfakis, Lauren; Kamaldinov, Tim; Khmaladze, Alexander; Hosseini, Zeinab F.; Nelson, Deirdre A.; Larsen, Melinda; and Castracane, James, "Mesenchymal Cells Affect Salivary Epithelial Cell Morphology on PGS/PLGA Core/Shell Nanofibers" (2018). Biological Sciences Faculty Scholarship. 10.
https://scholarsarchive.library.albany.edu/biology_fac_scholar/10