Date of Award




Document Type


Degree Name

Doctor of Philosophy (PhD)


Department of Chemistry

Content Description

1 online resource (iii, xiv, 77 pages) : illustrations (some color)

Dissertation/Thesis Chair

Igor K Lednev

Committee Members

Russell Gettig, Jan Halamek, Jayanti Pande, Alexander Shekhtman


Body Fluids, Chemometrics, Forensics, Multivariate Data Analysis, Spectroscopy, Raman spectroscopy, Body fluids, Identification, Mass spectrometry, Chemistry, Forensic

Subject Categories

Analytical Chemistry | Biochemistry | Chemistry


The challenges to forensic body fluid analysis have placed limitations on the type of information that investigators can acquire and how that information can be collected. In recent years, Raman spectroscopy has proven itself useful for characterizing body fluids. In 2008, a large-scale investigation was undertaken to explore the use of Raman spectroscopy as a means of identifying body fluids. This work resulted in multidimensional Raman spectroscopic signatures for the five main body fluids: semen, peripheral blood, saliva, vaginal fluid, and sweat. These studies were incredibly successful and created the foundation for years of continued research. Accordingly, the studies included in this thesis have been specifically chosen to frame the previous research projects. They include a suite of projects aimed to advance and validate the developed method.