Date of Award

1-1-2011

Language

English

Document Type

Dissertation

Degree Name

Doctor of Philosophy (PhD)

College/School/Department

Department of Biological Sciences

Content Description

1 online resource (xi, 116 pages) : illustrations (some color)

Dissertation/Thesis Chair

Hua Shi

Committee Members

Douglas Conklin, Min-Ho Lee, Richard Zitomer

Keywords

application, aptamer, design, intron homing, RNA, transcription activation, Introns

Subject Categories

Biochemistry | Biology | Molecular Biology

Abstract

aptamers are being developed as an essential tool in many fields of biological research. Their utility is not limited to being protein inhibitors; a lot of novel functions can be realized. However, in vivo application of RNA aptamers still faces many challenges. The aim of this dissertation is to design and apply composite aptamers in multiple expression and delivery systems to address some critical issues, such as correct folding, high level production, degradation by nucleases, excessive consumption of cellular resource and potential toxic effect.

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