"Development of ambient ionization mass spectrometry approaches for the" by Megan Isabella Chambers

Date of Award

1-1-2023

Language

English

Document Type

Dissertation

Degree Name

Doctor of Philosophy (PhD)

College/School/Department

Department of Chemistry

Content Description

1 online resource (xxxvii, 565 pages) : illustrations (some color)

Dissertation/Thesis Chair

Rabi A Musah

Committee Members

Igor Lednev, Ting Wang, Lori Ana Valentín

Keywords

Cannabis sativa, Direct analysis in real time - high-resolution mass spectrometry (DART-HRMS), Forensic science, Mass spectrometry of complex matrices, Psychoactive plant materials, Quantification of small molecules, Phytochemicals, Cannabis, Field ionization mass spectrometry, Chemistry, Forensic

Subject Categories

Chemistry

Abstract

Drug chemistry sections of forensic crime laboratories are tasked with analyzing a variety of unknown materials, a subset of which are derived from plants. In comparison to the analysis of small-molecule drugs, these plant matrices are incredibly complex and present unique challenges. When traditional chromatographic approaches are used to interrogate these materials in forensic investigations, large amounts of time, resources, and funds are directed towards the extensive sample preparation steps required. Exacerbating the issue is that the matrix characteristics often result in instrument downtime to accomplish the maintenance required to address ingredients adhering to columns or clogging syringes, which are common problems when testing Cannabis-derived samples. Furthermore, the increased legalization and decriminalization of marijuana in the U.S. has contributed to the rise in popularity of Cannabis products, which will require new testing methods to analyze the myriad of sample types available as the industry continues to evolve. Therefore, the unique capabilities of direct analysis in real time – high-resolution mass spectrometry (DART-HRMS) were investigated for the analysis of plant materials, including Cannabis, as an approach to circumventing many of the sample preparation and other difficulties often encountered.A DART-HRMS triage approach, which requires no sample pretreatment steps, has been developed for the detection of Cannabis-related molecules in a wide variety of sample types, including edibles, beverages, concentrates, topicals, vaporizers, marijuana/hemp flower, and pre-rolls. Additional applications of DART-HRMS for the analysis of Cannabis materials include the following: (1) differentiation of cannabinoids; (2) quantification of cannabinoids in edibles extracts; and (3) distinguishing between hemp and marijuana varieties of C. sativa through statistical analysis of mass spectral data. DART-HRMS was also proven effective for the analysis of plant-based legal-highs, specifically those identified as “plants of concern” by the United Nations Office on Drugs and Crime. Ambient ionization mass spectrometry (AIMS) approaches were developed for the rapid detection and validated quantification of psychoactive small-molecule constituents in complex plant matrices. The methods described would greatly improve forensic science and criminal justice practices in the U.S. for the analysis of complex plant materials, both Cannabis-derived and plant-based legal-highs.

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